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KMID : 0613820200300030291
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Journal of Life Science
2020 Volume.30 No. 3 p.291 ~ p.297
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Development and Genetic Diversity Analysis of Microsatellite Markers Using Next-generation Sequencing in Seriola quinqueradiata
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Dong Chun-Mae
Lee Mi-Nan
Kim Eun-Mi
Park Jung-Youn
Kim Kun-Do
Noh Jae-Koo
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Abstract
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This study was conducted to develop microsatellite markers in Seriola quinqueradiata using next-generation sequencing. A total of 28,873,374 reads were generated on an Illumina Hiseq2500 system, yielding 7,247,216,874 bp sequences. The de novo assembly resulted in 466,359 contigs. A total of 132 contigs (0.43%), including 60 microsatellite loci, were derived from 30,729 contigs longer than 518 bp. A total of 60 primer sets were designed from the 132 microsatellite loci. A total of 15 polymorphic nuclear microsatellite loci were chosen to evaluate population genetic parameters in the parents and offspring. The mean number of effective alleles was 18.5, ranging from 11 to 30. The observed heterozygosity (HO) and expected heterozygosity (HE) ranged between 0.431 and 0.972 with an average of 0.812 and from 0.782 to 0.949 with an average of 0.896, respectively. No significant linkage disequilibrium was observed after Bonferroni revision in any loci. The results show that the 15 polymorphic nuclear microsatellite markers can be used to study the population and conservation genetics of S. quinqueradiata in Korea. To ensure the success of artificial seedling production technology, genetic variations between the parent and offspring populations should be monitored, and inbreeding should be controlled.
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KEYWORD
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Artificial reproduction, genetic variability, microsatellite loci, next generation sequencing, Seriola quinqueradiata
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